Journal: Nature cell biology

Article Title: ATF4 couples MYC-dependent translational activity to bioenergetic demands during tumor progression

doi: 10.1038/s41556-019-0347-9

Figure Lengend Snippet: a. Immunoblot of B cells isolated from WT (n=3), pre-lymphoma Eμ-myc (n=3) or lymphoma bearing Eμ-myc mice (n=6), lysates from different mice per group were used. b . Immunoblot from two normal human B cells (NHBC) and Burkitt’s lymphoma cell lines. c. Immunoblot from normal human colonocytes (NHC) or colon cancer cell lines. d. Immunoblot from MCF10 (non-tumorigenic breast epithelial cell line) or breast cancer cell lines. e. Pearson correlation between EIF4EBP1 and ATF4 target gene expression in Diffused Large B cell Lymphoma (DLBCL, n=562), Colorectal Adenocarcinoma (COAD, n=329), Breast Cancer (BRCA, n=1218) and Sarcoma (SARC, n=265). The center lines depict linear regression lines and shaded regions are 95% confidence intervals for regression lines. Datasets analyzed are listed in the section. Previously known ATF4 target gene list used in this analysis is shown in table. f. Kaplan-Meier plots of progression free survival (left) and overall survival (right) of DLBCL patients with high or low EIF4EBP1 expression. The survival analysis using Kaplan-Meier and two-sided log-rank test between high and low EIF4EBP1 expression groups was performed on all the patients with records of progression-free survival (left, n= 173) and overall survival (right, n=171), respectively. g. Proposed model of ATF4 and MYC co-operation in tumor progression. All immunoblots are from n=1 and unprocessed scans of blots are shown in .

Article Snippet: Normal human colonocytes were purchased from Cell Applications Inc (732Cn-10a).

Techniques: Western Blot, Isolation, Targeted Gene Expression, Expressing

Journal: Experimental & Molecular Medicine

Article Title: Distribution and impact of p16 INK4A+ senescent cells in elderly tissues: a focus on senescent immune cell and epithelial dysfunction

doi: 10.1038/s12276-024-01354-4

Figure Lengend Snippet: a A schematic image illustrating how GzmA affects colonic epithelial cells through PARs signaling. b Overall scheme of the T cell isolation and senescence process (upper panel). Isolated pan-T cells after stimulation with anti-CD3/28 cocktail and rhIL-2 were assessed for cumulative population doubling level (PDL) (lower panel). Young cells were defined as 2 < cPDL < 5 and senescent cells as cPDL > 9. c The C 12 FDG staining analysis using flow cytometry (upper panels). The mRNA expression level of representative T cell senescence markers (p21 Waf1 , p16 INK4A , CD28, and CD57) are shown (lower panel). ND not detected. d The mRNA (upper panel) and protein (lower panel) levels of GzmA in young and senescent T cells are performed by real-time PCR and ELISA, respectively. e The IHC analysis of cleaved caspase-3 in normal colon tissues from young and elderly individuals (upper panel) and 4-month and 18-month-old mouse colon tissues (lower panel). The right panels display the quantification data, respectively. f , g Human primary colon epithelial cells were co-cultured with young or senescence T cells ( f ) or treated with CM of young or senescence T cells ( g ) for 3 days. The luminescence-based assay and ICC to determine cleaved caspase-3/7 activity and cleaved caspase-3 expression level were performed, respectively. h IHC analysis for IL8 was performed in normal colon from young and elderly tissues (upper panel). The IHC analysis results are categorized into “Low,” “Moderate,” and “High” based on the intensity of the immunostaining, with representative images provided for each category. The left lower panel shows the quantification data for IHC analysis. The right lower panel shows a dot plot illustrating the expression of CXCL8 (IL8) in colonic epithelial cells from young and elderly individuals in the GSE178341 data set. i The CXCL8 (IL8) mRNA expression was analyzed by real-time PCR in human colon epithelial cells, which were co-cultured with young or senescent T cells (upper panel) and treated with CM of young or senescent T cells (lower panel) for 3 days. A p -value in ( h ) is calculated using the Chi-square test. The rest of the p -values are calculated using the Mann–Whitney U test. The graph in ( b ) is shown as mean ± standard deviation, while the rest of the bar graphs are shown as mean + standard deviation. “Young” and “Sen” in ( c , d , f , g , i ) indicate the young and senescent T cells, respectively. “Young” and “Old” in ( e , h ) indicate the young and the elderly individuals, respectively.

Article Snippet: These mixtures were treated to human colonic epithelial cells (HCoEpiC, Cell Biologics, Inc.) for 1 day.

Techniques: Cell Isolation, Isolation, Staining, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Cell Culture, Luminescence Assay, Activity Assay, Immunostaining, MANN-WHITNEY, Standard Deviation

Journal: Experimental & Molecular Medicine

Article Title: Distribution and impact of p16 INK4A+ senescent cells in elderly tissues: a focus on senescent immune cell and epithelial dysfunction

doi: 10.1038/s12276-024-01354-4

Figure Lengend Snippet: A schematic image illustrating how senescent T cells can affect colonic epithelial function via the PARs signaling pathway.

Article Snippet: These mixtures were treated to human colonic epithelial cells (HCoEpiC, Cell Biologics, Inc.) for 1 day.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Toxicity Assessment of Wild Mushrooms from the Western Ghats, India: An in Vitro and Sub-Acute in Vivo Study

doi: 10.3389/fphar.2018.00090

Figure Lengend Snippet: (A) The effect of the extracts on the morphology of NCM460 cells as visualized under a phase contrast microscope. (B) IC 50 values of the extracts. (C) Dose response curves w.r.t. exposure to different concentrations of the mushroom extracts on the intestinal cells. Data presented are the means ± SD of results from three independent experiments. “#” represents significant difference with respect to AB at p < 0.05.

Article Snippet: The NCM460 (colon epithelial cells) and Chang's liver cell lines were obtained from National Centre for Cell Sciences (NCCS), Pune, India, and grown at 37°C in a humidified atmosphere of 5% CO 2 using MEM, supplemented with 10% FBS and antibiotic solution.

Techniques: Microscopy

Journal: ACS applied materials & interfaces

Article Title: Design, Synthesis, and Nanostructure-Dependent Antibacterial Activity of Cationic Peptide Amphiphiles

doi: 10.1021/acsami.8b17808

Figure Lengend Snippet: Cytotoxicity of designed PA against HaCaT, HCEC and HEK-293 and hemolytic activity against Human red blood cells.

Article Snippet: Immortalized Human Keratinocyte (HaCaT) cell line was donated by Dr. David Oupicky Lab (Center for Drug Delivery and Nanomedicine – UNMC) and Immortalized Human Colon Epithelial Cell (HCEC) line was donated by Dr. Robert Lewis Lab (Eppley Cancer Institute – UNMC).

Techniques: Activity Assay

Journal: Genes & Genomics

Article Title: Tumor suppressive effect of scavenger receptor class A member 5 overexpression in colorectal cancer by regulating PI3K/AKT/mTOR pathway

doi: 10.1007/s13258-021-01139-3

Figure Lengend Snippet: Scavenger receptor class A member 5 (SCRAR5) expression in colorectal cancer (CRC) cells. The mRNA and protein levels of SCRAR5 in CRC cell lines (HT29, HCT116, SW480, and SW620) was significantly lower than that in normal HCoEpic cell line by qPCR ( A ) and western blotting ( B ), respectively

Article Snippet: Human normal colonic epithelial cell line HCoEpic and four CRC cell lines, including SW620, SW480, HT29, and HCT116, were purchased from Nanjing KeyGen Biotech.

Techniques: Expressing, Western Blot